發(fā)布時(shí)間：2018-02-26 22:46

  本文關(guān)鍵詞： 胚胎干細(xì)胞 肝細(xì)胞 組織工程 干細(xì)胞 臍帶臍血干細(xì)胞 小鼠胚胎干細(xì)胞 肝組織定向分化 分化階段 UGTa UGTa mG ST 表達(dá)特征 胚層結(jié)構(gòu)　出處：《中國(guó)組織工程研究》2016年28期 　論文類型：期刊論文

【摘要】：背景:目前對(duì)于小鼠胚胎干細(xì)胞不同分化階段的尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a1、尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a6和微粒體谷膚甘膚S-轉(zhuǎn)移酶1表達(dá)特征缺乏研究,報(bào)道較少。目的:觀察小鼠胚胎干細(xì)胞不同分化階段普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a1、尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a6和微粒體谷膚甘膚S-轉(zhuǎn)移酶1的表達(dá)特征。方法:分離培養(yǎng)Wistar大鼠胚胎成纖維細(xì)胞,制備飼養(yǎng)層細(xì)胞。將胚胎干細(xì)胞接種于飼養(yǎng)層細(xì)胞上,誘導(dǎo)胚胎干細(xì)胞分化為肝細(xì)胞,采用Western blot檢測(cè)尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a1、尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a6和微粒體谷膚甘膚S-轉(zhuǎn)移酶1表達(dá)特征,色譜法測(cè)定計(jì)算微粒體谷膚甘膚S-轉(zhuǎn)移酶1催化活性。結(jié)果與結(jié)論:在胚胎干細(xì)胞在分化為肝細(xì)胞過程中,尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a1呈現(xiàn)上升趨勢(shì);尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a6在分化起初并未發(fā)現(xiàn)其表達(dá),而在分化第18天后表達(dá)相對(duì)較高;微粒體谷膚甘膚S-轉(zhuǎn)移酶1在胚胎干細(xì)胞分化為干細(xì)胞整個(gè)過程中均具有較高的表達(dá),但表達(dá)豐度均低于成年小鼠干細(xì)胞。干細(xì)胞分化第18天,肝組織微粒存在微粒體谷膚甘膚S-轉(zhuǎn)移酶1催化活性為7.65μmol/(min·g)。結(jié)果表明,胚胎干細(xì)胞分化為肝細(xì)胞過程中,尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a1相對(duì)比較穩(wěn)定,尿普二磷酸葡萄糖醛酞轉(zhuǎn)移酶1a6在分化不同過程中呈現(xiàn)出上升趨勢(shì),而微粒體谷膚甘膚S-轉(zhuǎn)移酶1在胚胎干細(xì)胞分化為肝細(xì)胞過程中表達(dá)甚微。
[Abstract]:Background: at present, there is a lack of research on the expression characteristics of UDP glucosaldehyde-phthaletransferase 1a1, UDP glucosaldehyde-phthaletransferase 1a6 and microsomal gluconectin S-transferase 1 in different differentiation stages of mouse embryonic stem cells. Objective: to observe the expression characteristics of Glucosyltransferase 1a1, UDP 1a6 and microsomal glucomannin S-transferase 1 at different differentiation stages of mouse embryonic stem cells. Methods: Wistar rat embryonic fibroblasts were isolated and cultured. To prepare feeder layer cells. Embryonic stem cells were inoculated on feeder layer cells to induce embryonic stem cells to differentiate into hepatocytes. Western blot was used to detect the expression of urinary glucosylaldehyde-transferase (1a1), glucosyltransferase (1a6) and microsomal glucosyltransferase 1 (S-transferase 1). Results and conclusion: in the process of differentiation of embryonic stem cells into hepatocytes, urine phosphoglucomaldehyde-phthaletransferase 1a1 showed an increasing trend. At the beginning of differentiation, the expression of UDP Glucotransferase 1a6 was not found, but the expression was relatively high at the 18th day after differentiation. Microsomal SS-transferase 1 was highly expressed during the differentiation of embryonic stem cells into stem cells, but the expression abundance was lower than that of adult mouse stem cells. The catalytic activity of microsomal glutenin S-transferase 1 in liver tissue was 7.65 渭 mol/(min 路gr. The results showed that the urine gluconaldehyde diphosphate phthaletransferase 1a1 was relatively stable during the differentiation of embryonic stem cells into hepatocytes. UDP glucosaldehyde-phthaletransferase 1a6 showed an upward trend in different differentiation processes, while microsomal glucomannin S-transferase 1 expressed very little in the differentiation of embryonic stem cells into hepatocytes.
【作者單位】： 南通大學(xué)醫(yī)學(xué)院;
【分類號(hào)】：R575

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