發(fā)布時(shí)間：2018-08-08 14:28

【摘要】：從實(shí)驗(yàn)的角度探討了熒光產(chǎn)生效率與溫度的依賴關(guān)系,實(shí)驗(yàn)中用不同波長激發(fā)光在不同溫度下激發(fā)血清,研究了血清熒光強(qiáng)度隨溫度的變化規(guī)律。實(shí)驗(yàn)結(jié)果表明:當(dāng)溫度在28~33℃范圍內(nèi)變化時(shí),激發(fā)的血清熒光強(qiáng)度隨著溫度的增加熒光強(qiáng)度減小。當(dāng)溫度在35~41℃范圍內(nèi)變化時(shí),熒光強(qiáng)度隨溫度增加不再是線性減小,而是出現(xiàn)跳躍式變化。當(dāng)用320~350nm激發(fā)光激發(fā)血清時(shí),隨著溫度的增加熒光強(qiáng)度接近線性減小。當(dāng)用其它波長的光激發(fā)血清時(shí),熒光強(qiáng)度并不隨溫度線性地減小,研究為探測血清熒光光譜時(shí)樣品的溫度選擇提供了實(shí)驗(yàn)依據(jù)。
[Abstract]:From the point of view of experiment, the dependence of fluorescence production efficiency on temperature was discussed. In the experiment, different wavelengths of excitation light were used to excite the serum at different temperatures, and the variation of serum fluorescence intensity with temperature was studied. The experimental results show that the fluorescence intensity of the stimulated serum decreases with the increase of temperature when the temperature varies in the range of 2833 鈩，

本文編號：2172077