【摘要】：目的探討深圳兒童血清微量元素鈷水平與手足口病感染的關(guān)系。方法于2013-2015年間在深圳市兒童醫(yī)院采集手足口病重癥病例(202例)、輕癥(220例)和在該院體檢的健康兒童(208例)的咽拭/肛拭樣本及血樣。采用實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(polymerase chain reaction,PCR)檢測病原體類型。用水浴酸消化-電感耦合等離子體質(zhì)譜法測定血清中鈷含量,并進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果對(duì)照組、輕癥組與重癥組兒童血清微量元素鈷含量分別為0.36μg/kg、0.40μg/kg和0.31μg/kg。重癥組鈷含量低于輕癥組和對(duì)照組(均有P0.05),輕癥組與對(duì)照組間鈷含量差異無統(tǒng)計(jì)學(xué)意義(Z=-0.821,P=0.412)。鈷與手足口病病情呈中度負(fù)相關(guān)(r=-0.298,P0.001)。血清鈷水平在性別間、年齡間差異均無統(tǒng)計(jì)學(xué)意義(均有P0.05)。不同病毒感染類型(EV71型、CA16型、其他)間血清鈷含量均有差異,其中輕癥組中EV71型患兒血清鈷水平高于CA16型,差異有統(tǒng)計(jì)學(xué)意義(Z=4.397,P0.001)。重癥組中EV71型與CA16型患兒血清鈷水平差異無統(tǒng)計(jì)學(xué)意義(Z=-1.821,P=0.069)。同時(shí)Logistic回歸分析結(jié)果顯示隨著一定范圍內(nèi)血清鈷濃度的升高,患手足口病風(fēng)險(xiǎn)降低。結(jié)論兒童血清微量元素鈷含量可能對(duì)兒童感染手足口病有一定作用。
[Abstract]:Objective to investigate the relationship between serum trace element cobalt level and hand, foot and mouth disease infection in Shenzhen children. Methods from 2013 to 2015, pharynx swabs / anal swabs and blood samples were collected from 202 severe cases of hand, foot and mouth disease (202 cases), 220 cases of mild hand, foot and mouth disease (220 cases) and 208 healthy children (208 cases) examined in Shenzhen Children's Hospital. Real-time fluorescence quantitative polymerase chain reaction (polymerase chain reaction,PCR) was used to detect pathogen types. The content of cobalt in serum was determined by water bath acid digestion and inductively coupled plasma mass spectrometry, and the statistical analysis was carried out. Results the serum trace elements cobalt content of children in control group, mild group and severe group were 0.36 渭 g / kg, 0.40 渭 g / kg and 0.31 渭 g / kg., respectively. The cobalt content in the severe group was lower than that in the mild group and the control group (P 0.05). There was no significant difference in cobalt content between the mild group and the control group (Z 鈮，

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