本文選題：煙粉虱 + 性別決定　； 參考：《中國(guó)農(nóng)業(yè)科學(xué)院》2016年博士論文

【摘要】：性別決定是生物兩性形成和正常生長(zhǎng)發(fā)育的基礎(chǔ),對(duì)種群延續(xù)意義重大。煙粉虱是一種世界性農(nóng)業(yè)害蟲(chóng),為單雙倍體昆蟲(chóng)。雄蟲(chóng)來(lái)自未受精的卵為單倍體,雌蟲(chóng)來(lái)自受精卵為雙倍體。為了研究煙粉虱的性別決定機(jī)制,對(duì)其性別決定中的兩個(gè)關(guān)鍵基因transformer2(Bttra2)和doublesex(Btdsx)進(jìn)行了克隆和分析,并進(jìn)一步利用RNAi技術(shù)闡述了其在煙粉虱性別決定中的作用。研究結(jié)果表明煙粉虱Bttra2全長(zhǎng)1702bp,由10個(gè)外顯子和9個(gè)內(nèi)含子組成;Btdsx全長(zhǎng)2339bp,由7個(gè)外顯子和6個(gè)內(nèi)含子組成。發(fā)現(xiàn)Bttra2有四種剪接形式,編碼四種蛋白,表達(dá)豐度雄蟲(chóng)高于雌蟲(chóng);而B(niǎo)tdsx有13種剪接形式,編碼六種蛋白,表達(dá)豐度雄蟲(chóng)也高于雌蟲(chóng)。明確了transformer2和Btdsx在煙粉虱性別決定通路中均發(fā)揮至關(guān)重要作用且兩者相互影響和制約,Bttra2/Btdsx基因的干擾影響Vg基因和Btdsx/Bttra2的表達(dá)量,造成煙粉虱雄蟲(chóng)生殖器官尾針畸形。研究結(jié)果對(duì)于闡明煙粉虱性別決定機(jī)制具有重要理論意義。
[Abstract]:Sex determination is the basis for the formation of biological hermaphroditism and normal growth and development, which is of great significance for population continuation. Bemisia Tabaci is a worldwide agricultural pest, a haploidy insect. Males from unfertilized eggs were haploid and females from fertilized eggs were diploidy. In order to study the sex determination mechanism of Bemisia Tabaci, two key genes, transformer2Bttra2) and DoublesexexBtdsx), were cloned and analyzed, and their roles in sex determination of Bemisia Tabaci were further elucidated by using RNAi technique. The results showed that the total length of Bemisia Tabaci Bttra2 was 1702 BP, which consisted of 10 exons and 9 introns, which consisted of 7 exons and 6 introns. It was found that Bttra2 had four splicing forms, encoding four proteins, and that Btdsx had 13 splicing forms, encoding six proteins, and expressed more abundant male than female. It is clear that transformer2 and Btdsx play an important role in the sex-determining pathway of Bemisia Tabaci and that the interference of Bttra2 / Btdsx gene affects the expression of Vg gene and Btdsx/Bttra2 and causes malformation of male reproductive organ of Bemisia Tabaci. The results are of great theoretical significance to clarify the sex determination mechanism of Bemisia Tabaci (Bemisia Tabaci).
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)科學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：S433

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1 郭利桃;基于transformer2和doublesex級(jí)聯(lián)互作的煙粉虱性別決定機(jī)制研究[D];中國(guó)農(nóng)業(yè)科學(xué)院;2016年

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本文編號(hào)：1857775