Grain size is a major determinant of cereal grain yields; however, the relevant regulatory mechanisms controlling this trait have not been fully elucidated. The rice(Oryza sativa) mutant short grain6(sg6) was identified based on its reduced grain length and weight. Here, we functionally characterized the role of SG6 in determining grain size through the regulation of spikelet hull cell division. SG6 encodes a previously uncharacterized plant ATrich sequence and zinc-binding(PLATZ) protein that i...

【文章頁數(shù)】：18 頁

【文章目錄】：
INTRODUCTION
RESULTS
    sg6 produces shorter grains
    SG6 encodes a novel PLATZ protein,and its overexpression significantly enlarges seed size and weight
    SG6 regulates the cell division of the spikelet hulls
    SG6 is preferentially expressed in the panicles
    SG6 promotes the expression of cell‐cycle‐related genes
    SG6 interacts with putative cell proliferation‐related regulators
DISCUSSION
    SG6 is a novel and important regulator of grain size
    SG6 regulates the cell cycle
    PLATZ family proteins are functionally diverse
MATERIALS AND METHODS
    Plant materials and growth conditions
    Constructs and phenotype analysis
    RNA extraction and real‐time PCR analysis
    Promoter‐GUS fusion study
    Subcellular localization study
    Yeast two hybrid analysis
    Histological analysis
    RNA‐seq analysis
    Flow cytometric analysis
    Accession numbers
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



本文編號：3991277