雙生病毒是一類重要的植物病毒,病毒粒子呈典型的孿生狀,基因組由單鏈環(huán)狀DNA構(gòu)成。當前,雙生病毒科被分為9個屬,分別為Becurtovirus,Begomovirus,Capulavirus,Curtovirus,Eragrovirus,Grablovirus,Mastrevirus,Topocuvirus 和 Turncurtovirus。雙生病毒,特別是begomovirus,在熱帶和亞熱帶地區(qū)嚴重危害。在一些欠發(fā)達國家和地區(qū),它們甚至會造成饑荒。近年來,begomovirus的發(fā)生在中國南方越來越普遍。然而,這類病毒在福建省的發(fā)生情況還不甚清楚。在第一部分,本研究于2015-2017年間,從福建各地采集了疑似begomovirus侵染的朱槿、玫瑰茄、黃秋葵、黃麻、芒麻、番茄和勝紅薊等植物樣本。利用簡并引物PCR并結(jié)合必要的全基因組克隆,本研究從這些樣本中鑒定到6種begomovirus,分別為木爾坦棉花曲葉病毒（CLCuMuV）、番茄黃化曲葉病毒（TYLCV）、越南黃麻黃脈病毒（COYVV）,苧麻花葉病毒（RamV）、中國勝紅薊黃脈病毒（AYVCnV）和野葛花葉病毒（KuMV）。... 

【文章來源】：福建農(nóng)林大學(xué)福建省

【文章頁數(shù)】：115 頁

【學(xué)位級別】：博士

【文章目錄】：
List of Abbreviations
摘要
Abstract
Chapter 1 Introduction and Review of literature
    1.1 Geminiviruses: General description
    1.2 Geminiviruses:Txonmy
    1.3 Geminiviruses: Genome organization
    1.4 Geminiviruses: gene function and host-virus interaction
    1.5 Geminiviruses: Ecology
    1.6 Geminiviruses in China and Fujian Province
    1.7 Objectives of Research
Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina
    2.1 Background
    2.2 Materials and methods
        2.2.1 Collection of samples
        2.2.2 Genomic DNA extraction
        2.2.3 PCR amplification, cloning, and sequencing
        2.2.4 PCR/RCA amplification of DNA P
        2.2.5 Phylogenetic analysis
    2.3 Results and Discussion
        2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China
            2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa
            2.3.1.2 Detection of Ramie mosaic virus （RamV） from Ramie （Boehmeria nivea）
            2.3.1.3 Detection of Tomato yellow leaf curl virus （TYLCV） from Tomato plants（Lycopersicon esculentum L）
            2.3.1.4 Detection of Corchorus yellow vein Vietnam virus （CoYVV） from Jute plants（Corchorus olitorius L.）
            2.3.1.5 Detection of Ageratum yellow vein China virus （AYVCnV） infecting Ageratum（Ageratum conyzoides）
            2.3.1.6 Detection of Kudzu mosaic virus virus （KuMV） infecting Kudzu （Ageratumconyzoides）
Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV
    3.1 Background
    3.2 Material and Methods
        3.2.1 Collection of samples and DNA extraction
        3.2.2 Construction of infectious clone and agrobacterium infilteration
        3.2.3 Confirmation of infection by Southern Blot analysis
        3.2.4 Cloning and sequence analysis:
    3.3 Results and Discussion
Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses
    4.1 Background
    4.2 Material and Methods
        4.2.1 Virus Source and inoculation
        4.2.2 Detection of TSSs by Southern Blot analysis
        4.2.3 Primer Design
        4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP
    4.3 Results and Discussion
        4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus（CLCuMuV）, Ramie mosaic virus （RamV）, Corchorus yellow vein Vietnam virus（CoYVV）
        4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs
Chapter 5 General Discussion
Chapter 6 Conclusions and Future perspective of this study
    6.1 Conclusion
    6.2 Future Perspective
References
Published Papers and other Academic Achievements during the Study
Acknowledgement
Appendices
    Appendix A
    Appendix B


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期刊論文
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