發(fā)布時(shí)間：2018-01-08 10:02

  本文關(guān)鍵詞：N-乙酰半胱氨酸對(duì)膿毒癥大鼠腸損傷的保護(hù)作用及對(duì)炎癥因子表達(dá)的影響　出處：《沈陽(yáng)農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年02期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： N-乙酰半胱氨酸 膿毒癥 大鼠 腸粘膜

【摘要】：為探討N-乙酰半胱氨酸(NAC)對(duì)膿毒血癥大鼠腸損傷的影響及對(duì)炎癥因子表達(dá)的影響,選取清潔級(jí)SD大鼠30只,體重250~300g,隨機(jī)分為假手術(shù)組(Sham組)、膿毒癥組(SEP組)和N-乙酰半胱氨酸組(NAC組),每組10只;Sham組僅開(kāi)腹,暴露盲腸,NAC組和SEP組行盲腸結(jié)扎穿孔術(shù),再分別靜脈給予150mg·kg~(-1)NAC和等量生理鹽水;3組均于再灌注4h處死大鼠,觀察小腸組織病理學(xué)結(jié)果;ELISA檢測(cè)血中腸脂肪酸結(jié)合蛋白(I-FABP)和D-乳酸濃度;ELISA法和Western blot法檢測(cè)血清和小腸中TNF-α、IL-6、IL-10濃度。結(jié)果表明:光鏡下Sham組未出現(xiàn)病理?yè)p傷,SEP組損傷重,NAC組損傷減輕;與Sham組比較,SEP組I-FABP、D-乳酸顯著升高(p0.05);與SEP組比較,NAC組I-FABP、D-乳酸顯著降低(p0.05);與Sham組比較,SEP組血清中TNF-α、IL-6、IL-10顯著升高(p0.05);與SEP組比較,NAC組TNF-α、IL-6顯著降低(p0.05),IL-10顯著升高(p0.05),小腸炎癥相關(guān)因子變化趨勢(shì)同血清檢測(cè)。N-乙酰半胱氨酸對(duì)膿毒血癥大鼠腸黏膜屏障有保護(hù)作用,其機(jī)制與抑制炎癥反應(yīng)有關(guān)。
[Abstract]:To investigate the effects of N-acetylcysteine (NACN) on intestinal injury and the expression of inflammatory cytokines in sepsis rats, 30 SD rats of clean grade were selected, weighing 250 ~ 300g. They were randomly divided into sham-sham group, septic group (SEP group) and N-acetylcysteine group (NAC group, n = 10 in each group). In the Sham group, the caecum ligation and perforation were performed in the exposed caecum group and the SEP group, and were given 150 mg 路kg~(-1)NAC and the same amount of normal saline respectively. All the rats in the 3 groups were killed at 4 h after reperfusion, and the histopathological results of small intestine were observed. The concentrations of serum fatty acid binding protein (I-FABP) and D-lactic acid were detected by ELISA. ELISA and Western blot were used to detect the concentration of IL-10 in serum and small intestine. The results showed that there was no pathological injury in Sham group under light microscope. The damage of SEP group was alleviated. Compared with Sham group, I-FABPnD-lactic acid in SEP group was significantly higher than that in Sham group (P 0.05). Compared with SEP group, I-FABP- D- lactic acid in NAC group was significantly lower than that in SEP group. Compared with the Sham group, the serum TNF- 偽 IL-6 + IL-10 in the Sham group was significantly higher than that in the Sham group (P 0.05). Compared with SEP group, TNF- 偽 IL-6 in NAC group decreased significantly (P 0.05) and IL-10 increased significantly (p 0.05). The change of inflammatory related factors in small intestine is related to the protective effect of serum. N-acetylcysteine on intestinal mucosal barrier in rats with sepsis, and its mechanism is related to the inhibition of inflammatory reaction.
【作者單位】： 中國(guó)醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物部;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81473625)
【分類(lèi)號(hào)】：R459.7
【正文快照】： 膿毒癥(sepsis)是臨床常見(jiàn)的危急重癥之一,許多疾病病理生理學(xué)改變都會(huì)引起膿毒血癥,其本質(zhì)是機(jī)體由于感染等因素致使大量炎癥因子釋放,引發(fā)全身炎癥反應(yīng)綜合癥(systemic inflmmnatory response syndrome,SIRS),導(dǎo)致機(jī)體自身免疫性損傷。膿毒癥的發(fā)病機(jī)理復(fù)雜,不同的病因其機(jī)

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