本文選題：棕尾別麻蠅 + 蛹　； 參考：《中國(guó)寄生蟲學(xué)與寄生蟲病雜志》2017年03期

【摘要】：目的探討棕尾別麻蠅(Boettcherisca peregrine)蛹3個(gè)時(shí)期轉(zhuǎn)錄組的差異表達(dá),為進(jìn)一步探究棕尾別麻蠅蛹各個(gè)時(shí)期轉(zhuǎn)錄組變化提供實(shí)驗(yàn)數(shù)據(jù)。方法提取棕尾別麻蠅蛹3個(gè)時(shí)期第1(MY1)、5(MY2)和10天(MY3)的蠅蛹總RNA,反轉(zhuǎn)錄成c DNA,并進(jìn)行測(cè)序,使用軟件edge R對(duì)基因差異表達(dá)進(jìn)行分析,對(duì)差異基因進(jìn)行GO分類統(tǒng)計(jì)和富集分析;對(duì)已被注釋的基因單基因簇(Unigene)進(jìn)行KEGG代謝通路分析;使用MISA對(duì)Unigene進(jìn)行簡(jiǎn)單重復(fù)序列(SSR)檢測(cè)。結(jié)果蠅蛹3個(gè)時(shí)期c DNA經(jīng)基因差異表達(dá)分析,得到各時(shí)期樣品基因組表達(dá)水平,共捕獲38 727條Unigenes,將不同時(shí)期的蠅蛹轉(zhuǎn)錄組進(jìn)行兩兩比較,其差異表達(dá)基因數(shù)介于1 263~2 430,差異基因總數(shù)為5 283。聚類圖型得到MY1、MY2和MY3組的兩個(gè)樣品表達(dá)模式相似,聚為一支。棕尾別麻蠅蛹轉(zhuǎn)錄組共有8 856個(gè)Unigenes被注釋,被注釋到的代謝通路有336個(gè)。SSR查找發(fā)現(xiàn),從8 645個(gè)Unigenes中共查找到12 146個(gè)SSR位點(diǎn),占Unigene總數(shù)的22.3%。其中,單核苷酸重復(fù)所占比例最高,達(dá)到69.2%,其次是三核苷酸重復(fù)(21.0%)。SSR不同重復(fù)基元類型中,出現(xiàn)頻率最高的為A/T,其次是AAC/GTT、AG/GT。結(jié)論棕尾別麻蠅蛹在不同時(shí)期基因表達(dá)有顯著差異。
[Abstract]:Objective to investigate the differential expression of transcriptional groups in the pupae of Boettcherisca peregrine in order to provide experimental data for further study of the transcriptional changes in the pupae. Methods the total RNAs of the pupae were extracted from the pupae of Magnolia melanogaster in the 1st and 10th day. The total RNAs were inverted into c DNA and sequenced. The differentially expressed genes were analyzed by software edge R, and the differential genes were classified and analyzed by go classification and enrichment analysis. KEGG metabolic pathway analysis was carried out on the annotated single gene cluster Unigene.MISA was used to detect the simple repeat sequence (SSRs) of Unigene. Results the genomic expression levels of three stages of fly pupae were obtained by gene differential expression analysis. A total of 38,727 Unigenes were captured, and the transcriptional groups of pupae were compared. The number of differentially expressed genes was between 1263 and 2430, and the total number of differentially expressed genes was 5 283. The expression patterns of two groups of MY1, MY2 and MY3 were similar and clustered into one branch. A total of 8,856 Unigenes were identified in the pupae transcriptome, 336 of the metabolic pathways were identified by SSR search and 12,146 SSR loci were identified from 8,645 Unigenes, accounting for 22.3% of the total number of Unigenes. Among them, the proportion of single nucleotide repeats was the highest (69.2%), followed by trinucleotide repeats 21.0%, SSR with the highest frequency of A / T, and AAC / GTT / AGR / GT with the highest frequency. Conclusion there are significant differences in gene expression in the pupae of Alternaria melanogaster at different stages.
【作者單位】： 貴州醫(yī)科大學(xué)法醫(yī)學(xué)院法醫(yī)司法鑒定中心;
【基金】：國(guó)家自然科學(xué)基金(No.81641082) 貴州省高校工程技術(shù)研究中心項(xiàng)目(黔科合KY字[2013]114號(hào)) 貴州省現(xiàn)代法醫(yī)司法鑒定工程研究中心(黔發(fā)改高技[2016]1345) 貴州省科技廳計(jì)劃基金(黔科合LH字[2016]7360)~~
【分類號(hào)】：D919.4

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