本文關(guān)鍵詞：利用單克隆抗體特異性敲除技術(shù)解析中藥藥效物質(zhì)基礎(chǔ)的新方法，由筆耕文化傳播整理發(fā)布。

利用單克隆抗體特異性敲除技術(shù)解析中藥藥效物質(zhì)基礎(chǔ)的新方法

New method for analyzing pharmacodynamic material basis of traditional Chinese medicines by using specific knockout technology with monoclonal antibodies

[1] [2] [3]

ZHAO Yan, QU Hui-hua, WANG Qing-guo（1. The School of Preclinical Medicine, Beijing University of Chinese Medcine, Beijing 100029, China; 2. Center of Scientific Research, Bei

[1]北京中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院,北京100029; [2]北京中醫(yī)藥大學(xué)科學(xué)實驗中心,北京100029; [3]北京中醫(yī)藥大學(xué)經(jīng)典方劑的應(yīng)用基礎(chǔ)研究創(chuàng)新團隊,北京100029

文章摘要：中藥藥效物質(zhì)基礎(chǔ)研究是實現(xiàn)中藥現(xiàn)代化的關(guān)鍵問題之一。作者將小分子單克隆抗體技術(shù)引入中藥藥效物質(zhì)基礎(chǔ)研究，利用中藥活性成分的單克隆抗體制備相應(yīng)的免疫親和色譜柱，實現(xiàn)從藥材或復(fù)方中特異性地“敲除”該化合物，而“原樣”保留其他成分在量和比例關(guān)系上不變，通過藥理藥效的比較研究，來明確揭示該成分與整體中藥或復(fù)方功能主治的關(guān)聯(lián)度。利用單克隆抗體特異性敲除技術(shù)解析中藥藥效物質(zhì)基礎(chǔ)，是符合中藥自身特點的藥效物質(zhì)基礎(chǔ)研究新方法，獲得的研究結(jié)果不僅有助于從全新的角度理解物質(zhì)基礎(chǔ)，而且有助于理解中藥單味藥內(nèi)部，或復(fù)方藥味之間“配伍”的現(xiàn)代科學(xué)意義。

Abstr：Study on pharmacodynamic material basis of traditional Chinese medicines is one of the key issues for the moderni-zation of traditional Chinese medicine. Having introduced the monoclonal antibody technology into the study on pharmacodynamic mate-rial basis of traditional Chinese medicines, the author prepared the immunoaffinity chromatography column by using monoclonal anti-bodies in active components of traditional Chinese medicines, so as to selectively knock out the component from herbs or traditional Chinese medicine compounds, while preserving all of the other components and keeping their amount and ratio unchanged. A compara-tive study on pharmacokinetics and pharmacodynamics was made to explicitly reveal the correlation between the component and the main purpose of traditional Chinese medicines and compounds. The analysis on pharmacodynamic material basis of traditional Chinese medi-cines by using specific knockout technology with monoclonal antibodies is a new method for study pharmacodynamic material basis in line with the characteristics of traditional Chinese medicines. Its results can not only help study material basis from a new perspective, but also help find the modem scientific significance in single herb or among compounds of traditional Chinese medicines.

文章關(guān)鍵詞：

Keyword：:pharmacodynamic material basis of traditional Chinese medicines small molecule monoclonal antibody immuno-affinity chromatography specific knockout

課題項目：國家自然科學(xué)基金項目（81373542,81274043）;教育部新世紀優(yōu)秀人才培養(yǎng)計劃項目（NCET-04-0140）

  本文關(guān)鍵詞：利用單克隆抗體特異性敲除技術(shù)解析中藥藥效物質(zhì)基礎(chǔ)的新方法，由筆耕文化傳播整理發(fā)布。