單寧酸對(duì)經(jīng)戊二醛固定的牛心包改性研究
發(fā)布時(shí)間：2018-08-07 13:11
【摘要】：目前商業(yè)上用于替換心臟瓣膜手術(shù)的人工心臟瓣膜主要兩大類：生物瓣膜和機(jī)械瓣膜。機(jī)械瓣膜耐久性高,組織相容性差,植入人體后需要長(zhǎng)期進(jìn)行抗凝血治療,否則易產(chǎn)生血栓。生物瓣血液動(dòng)力學(xué)性能好,血栓形成的發(fā)生率較低,但耐久性較差且容易發(fā)生鈣化。同種瓣膜作為一種生物瓣膜,其組織相容性良好,但來(lái)源有限且耐久性較差,這些缺點(diǎn)使同種瓣膜在臨床應(yīng)用上受到限制。異種生物瓣膜是臨床上應(yīng)用較廣泛的生物瓣膜,對(duì)生物組織進(jìn)行生化處理是目前國(guó)內(nèi)外對(duì)異種生物瓣膜的主要研究?jī)?nèi)容。交聯(lián)劑及其他處理方法的使用使生物瓣膜的耐久性有了較大的提高,生物材料的鈣化現(xiàn)象也得到較大的改善。1968年,使用戊二醛對(duì)生物材料進(jìn)行處理和保存,使生物材料在臨床應(yīng)用上得到突破性的進(jìn)展。經(jīng)戊二醛處理后,材料的耐久性大大提高,具有較高的機(jī)械強(qiáng)度,低免疫原性。但戊二醛處理后的材料存在細(xì)胞毒性,易鈣化、衰敗等缺點(diǎn)使生物材料的遠(yuǎn)期使用效果不佳。因此尋找一種更加理想的處理方案或交聯(lián)劑對(duì)生物材料進(jìn)行處理,從而制備出力學(xué)性能、組織相容性、血液相容性更加理想的心臟瓣膜變得尤為重要。制備異種瓣膜的生物材料有多種,例如豬心包或牛心包。其中牛心包是一種來(lái)源廣泛,低免疫原性,組織相容性較好的生物材料。最理想的交聯(lián)劑可以保存生物材料完整的組成成分和三維超微結(jié)構(gòu),并且提高材料的力學(xué)性能和生物學(xué)穩(wěn)定性,但所有的交聯(lián)劑都或多或少存在著缺陷。不同的交聯(lián)劑對(duì)生物材料影響有很大的差別。本文使用一種新型環(huán)保低毒的交聯(lián)劑聯(lián)合傳統(tǒng)的交聯(lián)劑戊二醛對(duì)心包進(jìn)行改性處理并在性能上進(jìn)行比較,希望構(gòu)建一種力學(xué)性能和生物學(xué)性能良好,具有良好生物相容性的異種生物瓣膜。主要研究?jī)?nèi)容如下： (1)采用不同濃度單寧酸聯(lián)合戊二醛對(duì)牛心包處理,并對(duì)不同濃度的單寧酸聯(lián)合戊二醛處理后的牛心包進(jìn)行理化性質(zhì)的評(píng)價(jià),確定單寧酸聯(lián)合戊二醛的最佳濃度。通過對(duì)外觀、厚度、含水量、接觸角、熱皺縮溫度、抗拉伸強(qiáng)度、彈性模量的測(cè)試和SEM掃描分析對(duì)單寧酸聯(lián)合戊二醛處理牛心包的效果進(jìn)行評(píng)價(jià),并與戊二醛進(jìn)行比較。經(jīng)單寧酸處理后,牛心包厚度增加,韌性增強(qiáng),呈褐色,接觸角變小,熱皺縮溫度提高,抗拉伸強(qiáng)度增大,彈性模量提高,證明牛心包經(jīng)單寧酸處理后材料的親水性能提高,熱力學(xué)穩(wěn)定性和力學(xué)性能得到改善。SEM掃描結(jié)果表明：牛心包經(jīng)3%單寧酸處理后,纖維排列緊密、規(guī)則優(yōu)于其他處理組。根據(jù)各組單寧酸處理牛心包后理化性質(zhì)的結(jié)果,3%為處理牛心包的最佳單寧酸濃度。 (2)采用3%單寧酸聯(lián)合戊二醛對(duì)牛心包進(jìn)行處理,并探究且生物化學(xué)穩(wěn)定性,由于單寧酸本身的澀性,能與蛋白質(zhì)結(jié)合,能使生物體內(nèi)原生質(zhì)凝固,本文針對(duì)單寧酸聯(lián)合戊二醛對(duì)牛心包處理后的膠原蛋白和彈性蛋白的含量變化,以及處理后牛心包的抗酶解能力進(jìn)行了探究。結(jié)果顯示,單寧酸聯(lián)合戊二醛處理后的牛心包膠原蛋白含量和彈性蛋白含量得到了保護(hù),與戊二醛組比較減少?gòu)椥缘鞍椎牧魇?與上文提到的彈性模量的提高結(jié)果吻合�？姑附饽芰σ诧@著增強(qiáng),證明單寧酸聯(lián)合戊二醛處理后的牛心包后在生物化學(xué)穩(wěn)定性方面得到改善。體外細(xì)胞毒性檢測(cè)和溶血性能檢測(cè)結(jié)果表明,單寧酸聯(lián)合戊二醛處理的牛心包與戊二醛處理的牛心包有較低的細(xì)胞毒性,溶血率較低。細(xì)胞毒性級(jí)數(shù)為1級(jí),幾乎無(wú)毒性,且溶血率較低。本實(shí)驗(yàn)結(jié)果證明,3%單寧酸聯(lián)合戊二醛處理的牛心包在力學(xué)性能,生物相容性方面得到了極大的改善。
[Abstract]:At present, there are two main types of artificial heart valves in commercial replacement of heart valve surgery: biological valves and mechanical valves. Mechanical valves have high durability and poor histocompatibility. They need long-term anticoagulant therapy after implantation, otherwise thrombus is easy to produce. The same valve, as a biological valve, is a biological valve with good histocompatibility, but limited source and poor durability. These defects have limited the clinical application of the same valve. The biological valve is a widely used biological valve, and the biochemical treatment of biological tissue is currently in the country. The main research content of the alien biological valve. The use of crosslinking agent and other treatment methods made the durability of biological valve improved greatly. The calcification of biological materials has been greatly improved in.1968 years. The use of glutaraldehyde to treat and preserve biological materials make biological materials breakthrough in clinical application. Progress. After treated with glutaraldehyde, the durability of the material is greatly improved, with high mechanical strength and low immunogenicity. However, the defects of the materials treated with glutaraldehyde have the disadvantages of cytotoxicity, easy calcification, decay and so on, so as to find a more ideal treatment scheme or cross-linking agent for biological materials. The heart valves are particularly important in preparing mechanical properties, histocompatibility, and ideal blood compatibility. There are many biological materials for the preparation of dissimilar valves, such as the porcine pericardium or the bovine pericardium. The bovine heart bag is a biological material with wide sources, low immunogenicity and good histocompatibility. The ideal crosslinker is the best possible agent. To preserve the complete composition and the three-dimensional ultrastructure of the biomaterials, and to improve the mechanical and biological stability of the materials, all the crosslinker has more or less defects. The effects of different crosslinker on biological materials are very different. Modifying the pericardium by modified glutaraldehyde and comparing its properties, we hope to construct a kind of biocompatible biological valve with good mechanical and biological properties and good biocompatibility. The main contents are as follows:
(1) use different concentration of tannic acid and glutaraldehyde to treat bovine pericardium, and evaluate the physical and chemical properties of different concentration of tannic acid and glutaraldehyde, and determine the optimum concentration of tannic acid and glutaraldehyde. Through the appearance, thickness, water content, contact angle, thermal shrinkage temperature, tensile strength and modulus of elasticity The effects of tannic acid and glutaraldehyde were evaluated and compared with glutaraldehyde, and compared with glutaraldehyde. After tannic acid treatment, the thickness of the cow's heart was increased, the toughness was increased, the contact angle was smaller, the temperature of the heat shrinkage was increased, the tensile strength increased and the modulus of elasticity increased, which proved that the cow heart bag was treated with tannic acid after treatment. The hydrophilic properties of the material were improved, the thermodynamic stability and mechanical properties were improved by.SEM scanning. The results showed that after 3% tannic acid treatment, the fiber was arranged closely and the rules were better than the other treatment groups. According to the results of the physicochemical properties of the heart bag treated with tannic acid, 3% was the best tannic acid concentration in the cow's heart bag.
(2) using 3% tannic acid and glutaraldehyde to treat the bovine heart bag, and explore and be biochemical stability. Because of the astringency of tannic acid itself, it can combine with protein to solidify the protoplasm of the organism. In this paper, the content changes of the collagen and elastin after the treatment of the bovine heart bag with tannic acid and glutaraldehyde and after treatment are treated. The anti enzymatic ability of the bovine pericardium was explored. The results showed that the content of Niu Xinbao collagen and the content of elastin after tannic acid combined with glutaraldehyde were protected, and the loss of elastin was reduced with the glutaraldehyde group, which was in agreement with the increase of the modulus of elasticity mentioned above. The biochemical stability of the bovine pericardium treated with ningic acid and glutaraldehyde was improved. The cytotoxicity test and hemolytic test in vitro showed that the bovine pericardium treated with tannic acid and glutaraldehyde had lower cytotoxicity and lower hemolysis rate. The cytotoxicity progression was 1 and almost non-toxic. The results of the experiment show that 3% tannic acid combined with glutaraldehyde has greatly improved the mechanical properties and biocompatibility.
【學(xué)位授予單位】：東華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R318.08
本文編號(hào)：2170128
[Abstract]:At present, there are two main types of artificial heart valves in commercial replacement of heart valve surgery: biological valves and mechanical valves. Mechanical valves have high durability and poor histocompatibility. They need long-term anticoagulant therapy after implantation, otherwise thrombus is easy to produce. The same valve, as a biological valve, is a biological valve with good histocompatibility, but limited source and poor durability. These defects have limited the clinical application of the same valve. The biological valve is a widely used biological valve, and the biochemical treatment of biological tissue is currently in the country. The main research content of the alien biological valve. The use of crosslinking agent and other treatment methods made the durability of biological valve improved greatly. The calcification of biological materials has been greatly improved in.1968 years. The use of glutaraldehyde to treat and preserve biological materials make biological materials breakthrough in clinical application. Progress. After treated with glutaraldehyde, the durability of the material is greatly improved, with high mechanical strength and low immunogenicity. However, the defects of the materials treated with glutaraldehyde have the disadvantages of cytotoxicity, easy calcification, decay and so on, so as to find a more ideal treatment scheme or cross-linking agent for biological materials. The heart valves are particularly important in preparing mechanical properties, histocompatibility, and ideal blood compatibility. There are many biological materials for the preparation of dissimilar valves, such as the porcine pericardium or the bovine pericardium. The bovine heart bag is a biological material with wide sources, low immunogenicity and good histocompatibility. The ideal crosslinker is the best possible agent. To preserve the complete composition and the three-dimensional ultrastructure of the biomaterials, and to improve the mechanical and biological stability of the materials, all the crosslinker has more or less defects. The effects of different crosslinker on biological materials are very different. Modifying the pericardium by modified glutaraldehyde and comparing its properties, we hope to construct a kind of biocompatible biological valve with good mechanical and biological properties and good biocompatibility. The main contents are as follows:
(1) use different concentration of tannic acid and glutaraldehyde to treat bovine pericardium, and evaluate the physical and chemical properties of different concentration of tannic acid and glutaraldehyde, and determine the optimum concentration of tannic acid and glutaraldehyde. Through the appearance, thickness, water content, contact angle, thermal shrinkage temperature, tensile strength and modulus of elasticity The effects of tannic acid and glutaraldehyde were evaluated and compared with glutaraldehyde, and compared with glutaraldehyde. After tannic acid treatment, the thickness of the cow's heart was increased, the toughness was increased, the contact angle was smaller, the temperature of the heat shrinkage was increased, the tensile strength increased and the modulus of elasticity increased, which proved that the cow heart bag was treated with tannic acid after treatment. The hydrophilic properties of the material were improved, the thermodynamic stability and mechanical properties were improved by.SEM scanning. The results showed that after 3% tannic acid treatment, the fiber was arranged closely and the rules were better than the other treatment groups. According to the results of the physicochemical properties of the heart bag treated with tannic acid, 3% was the best tannic acid concentration in the cow's heart bag.
(2) using 3% tannic acid and glutaraldehyde to treat the bovine heart bag, and explore and be biochemical stability. Because of the astringency of tannic acid itself, it can combine with protein to solidify the protoplasm of the organism. In this paper, the content changes of the collagen and elastin after the treatment of the bovine heart bag with tannic acid and glutaraldehyde and after treatment are treated. The anti enzymatic ability of the bovine pericardium was explored. The results showed that the content of Niu Xinbao collagen and the content of elastin after tannic acid combined with glutaraldehyde were protected, and the loss of elastin was reduced with the glutaraldehyde group, which was in agreement with the increase of the modulus of elasticity mentioned above. The biochemical stability of the bovine pericardium treated with ningic acid and glutaraldehyde was improved. The cytotoxicity test and hemolytic test in vitro showed that the bovine pericardium treated with tannic acid and glutaraldehyde had lower cytotoxicity and lower hemolysis rate. The cytotoxicity progression was 1 and almost non-toxic. The results of the experiment show that 3% tannic acid combined with glutaraldehyde has greatly improved the mechanical properties and biocompatibility.
【學(xué)位授予單位】：東華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R318.08
【參考文獻(xiàn)】
相關(guān)期刊論文 前9條
1 黃盛東 ,劉曉紅 ,楊立信 ,龔德軍 ,劉延玲 ,徐志云;血管內(nèi)皮細(xì)胞生長(zhǎng)因子基因轉(zhuǎn)染誘導(dǎo)大鼠骨髓基質(zhì)干細(xì)胞分化為內(nèi)皮細(xì)胞[J];第二軍醫(yī)大學(xué)學(xué)報(bào);2003年12期
2 余喜訊,陳槐卿;多聚環(huán)氧化合物處理的豬胸腔動(dòng)脈性能特征的研究[J];四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年05期
3 張文斌,吳漢江,胡廣偉,姚本棧,周藝群;牛心包衍生膜材料的制備及理化性能檢測(cè)[J];口腔醫(yī)學(xué)研究;2005年01期
4 李勝利,胡盛壽,周建業(yè);染料介導(dǎo)光氧化固定牛帶瓣頸靜脈的生物特性評(píng)價(jià)[J];生物醫(yī)學(xué)工程學(xué)雜志;2005年04期
5 李莉;陳健;余喜訊;;生物交聯(lián)劑京尼平改造生物性組織為組織工程化血管支架的實(shí)驗(yàn)[J];中國(guó)組織工程研究與臨床康復(fù);2007年35期
6 張寶仁,邢建洲;心臟瓣膜外科的發(fā)展與展望[J];中華胸心血管外科雜志;2005年04期
7 龍小毛,龔光甫,胡建國(guó),林輝;牛心包纖維支架細(xì)胞重建組織工程體外實(shí)驗(yàn)研究(英文)[J];中國(guó)現(xiàn)代醫(yī)學(xué)雜志;2004年21期
8 劉洋;孫國(guó)成;劉珊珊;譚紅梅;歐陽(yáng)輝;易定華;劉維永;;染料介導(dǎo)光氧化處理去細(xì)胞豬主動(dòng)脈瓣的生物學(xué)特性[J];中國(guó)胸心血管外科臨床雜志;2008年03期
9 陳翔;秦永文;;心臟瓣膜病介入治療進(jìn)展[J];心血管病學(xué)進(jìn)展;2012年03期
，本文編號(hào)：2170128
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