本文選題：成纖維細(xì)胞生長(zhǎng)因子 + 神經(jīng)元　； 參考：《中國(guó)組織工程研究》2017年06期

【摘要】：背景:神經(jīng)干細(xì)胞具有自我更新和多向分化的潛能,高比例誘導(dǎo)其向神經(jīng)元分化具有很大的應(yīng)用前景。目的:探討堿性成纖維細(xì)胞生長(zhǎng)因子-殼聚糖載體體外對(duì)神經(jīng)干細(xì)胞向神經(jīng)元分化的影響,及分化的神經(jīng)元與肌細(xì)胞共培養(yǎng)是否能形成突觸樣聯(lián)系。方法:純化后的神經(jīng)干細(xì)胞分別與單純殼聚糖、可溶性堿性成纖維細(xì)胞生長(zhǎng)因子及堿性成纖維細(xì)胞生長(zhǎng)因子-殼聚糖載體共培養(yǎng)。誘導(dǎo)7 d后,通過(guò)β-tubulinⅢ染色觀察向神經(jīng)元的分化;通過(guò)β-tubulinⅢ和Cha T染色觀察向膽堿能神經(jīng)元的分化;通過(guò)β-tubulinⅢ和MHC染色觀察和肌細(xì)胞之間的突觸聯(lián)系。結(jié)果與結(jié)論:堿性成纖維細(xì)胞生長(zhǎng)因子-殼聚糖載體組,分化形成神經(jīng)元的比例是74%,顯著高于其他2組,分化的神經(jīng)元與共培養(yǎng)的肌細(xì)胞能形成突觸樣聯(lián)系。提示堿性成纖維細(xì)胞生長(zhǎng)因子-殼聚糖載體體外能夠促進(jìn)神經(jīng)干細(xì)胞的分化,且分化形成的神經(jīng)元能與共培養(yǎng)的肌細(xì)胞形成突觸樣聯(lián)系。
[Abstract]:Background: neural stem cells have the potential of self-renewal and multi-differentiation. Aim: to investigate the effect of basic fibroblast growth factor-chitosan carrier on neural stem cell differentiation into neurons in vitro and whether co-culture of differentiated neurons and myocytes can form synaptic connections. Methods: purified neural stem cells were co-cultured with chitosan, soluble basic fibroblast growth factor and basic fibroblast growth factor-chitosan carrier respectively. After 7 days of induction, the differentiation to neurons was observed by 尾 -tubulin 鈪，

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