本文關(guān)鍵詞：生物材料表面微納結(jié)構(gòu)對(duì)成骨相關(guān)細(xì)胞的影響，由筆耕文化傳播整理發(fā)布。

生物材料表面微納結(jié)構(gòu)對(duì)成骨相關(guān)細(xì)胞的影響

The Effects of Micro- and Nano-Structured Biomaterial Surfaces on Osteogenetic-Related Cells

[1] [2] [3] [4]

LU Xiong, FENG Bo WENG Jie. LENG Yang （ 1. Key Laboratory of Advanced Technologies of Materials （ Ministry of Education） , School of Materials Science and Engineering, So

[1]西南交通大學(xué)材料科學(xué)與工程學(xué)院材料先進(jìn)技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室,四川成都610031; [2]香港科技大學(xué)機(jī)械工程系,香港999077

文章摘要：生物醫(yī)用材料表面性能，包括表面形貌與化學(xué)組成，對(duì)誘導(dǎo)骨組織形成并形成骨整合具有重要作用。細(xì)胞行為對(duì)基底表面形貌和組成的依賴性決定了設(shè)計(jì)不同功能表面的重要性。作者小組多年來從事生物材料表面微納結(jié)構(gòu)相關(guān)研究。在微圖形方面，結(jié)合微加工和磁控濺射技術(shù)制備出的羥基磷灰石微溝槽；采用溶膠一凝膠與復(fù)制微模塑相結(jié)合的方法制備了TiO2微圖形；采用掩模曝光電化學(xué)微加工技術(shù)和噴射電化學(xué)微加工技術(shù)，在鈦基底上制備多孔微圖形；通過轉(zhuǎn)移微模塑法與自組裝技術(shù)相結(jié)合，得到殼聚糖與牛血清蛋白復(fù)合微圖形。在納米結(jié)構(gòu)方面，采用電化學(xué)陽極氧化處理，獲得一定管徑和管長(zhǎng)的二氧化鈦納米管。在微納多級(jí)結(jié)構(gòu)方面，，結(jié)合高壓微弧氧化和低壓陽極氧化制備了微納多級(jí)結(jié)構(gòu)鈦表面。除了考慮微納結(jié)構(gòu)單獨(dú)效應(yīng)之外，還考慮了微納結(jié)構(gòu)化與生物功能化的協(xié)同效應(yīng)，即在具有微納結(jié)構(gòu)的生物材料表面通過層層自組裝等手段進(jìn)行生物化學(xué)修飾。最后通過成骨相關(guān)細(xì)胞培養(yǎng)實(shí)驗(yàn)及體內(nèi)植入實(shí)驗(yàn)，考察各試樣的生物活性。研究表明，微米尺度表面促進(jìn)骨細(xì)胞粘附、增殖、分化等，而納米尺寸結(jié)構(gòu)以及微納多級(jí)結(jié)構(gòu)對(duì)細(xì)胞功能具有進(jìn)一步促進(jìn)作用。微納結(jié)構(gòu)化與表面功能化修飾存在有協(xié)同效應(yīng)。這些研究結(jié)果為微納米技術(shù)應(yīng)用于人體植入研究提供了新方向。

Abstr：Surface properties including topography and chemistry are of great significance in deciding the response of tis- sue to implants. Our group has been engaged in researches on micro/nano structured biomaterial surfaces for a long time. This article reviews our series works on osteogenetic cells behavior on biomaterial surfaces with micro-and nano-struetures. For micro-patterns, hydroxyapatite microgrooves were prepared by combining micro-fabrication technology and magnetron sputtering technology; TiO2 micropatterns were obtained by combining sol-gel and replica molding; Micro-patterned Ti substrates were prepared by using a through mask electrochemical micromachining and a jet electrochemical micromachin- ing technology; chitosan/bovine serum albumin micropatterus were prepared on functionalized Ti surfaces by micro-trans- fer molding combined with self-assembly. For nanostruetures, titania nanotubes with various diameters and lengths were prepared by a electrochemical anodic oxidation treatment. For micro-nano hierarchical structures, titania micropores modi- fied with nanotubes were obtained by high voltage micro-arc oxidation and low voltage anodization. In addition to consider- ing the effects of micro-nano structure alone, the synergistic effects of struturalization and biofunctionalization of biomateri-al surfaces were investigated, which were realized through layer-by-layer self-assembly and other means of biochemical modification on micro/nano structured surfaces. Finally, in vitro osteogenetic cell culture and in vivo study were conducted to investigate the biological activity of various sample. The re- suits indicate that micro-scale topographical features promote cell adhesion, bone ingrowth and the formation of mechanical interlocking

文章關(guān)鍵詞：

Keyword：:Biomaterial surface osteogcnetic cell micro-structure nano-structure

課題項(xiàng)目：科技部“973”計(jì)劃項(xiàng)目（2012CB933602）;國(guó)家自然科學(xué)基金資助項(xiàng)目（31070851,51172188）;教育部新世紀(jì)優(yōu)秀人才基金（NCET-10-0704）;四川省杰出青年基金項(xiàng)目（2011JQ0010）;中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金（SWJTU11ZT11,SWJTU11CX150）

  本文關(guān)鍵詞：生物材料表面微納結(jié)構(gòu)對(duì)成骨相關(guān)細(xì)胞的影響，由筆耕文化傳播整理發(fā)布。