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一次性尿液采集器用于尿液HIV-1抗體檢測(cè)的實(shí)驗(yàn)室評(píng)估

發(fā)布時(shí)間:2018-05-25 07:11

  本文選題:一次性尿液采集器 + 尿液; 參考:《中國(guó)艾滋病性病》2017年01期


【摘要】:目的評(píng)估一次性尿液采集器保存的尿液樣本,用于艾滋病病毒(HIV)抗體檢測(cè)的可行性。方法利用尿液酶聯(lián)免疫吸附試驗(yàn),比較了普通樣品管和采集器對(duì)尿液HIV-1抗體檢出的影響;進(jìn)一步檢測(cè)了不同溫度和儲(chǔ)存時(shí)間下,采集器對(duì)尿液HIV-1抗體保存的均一性、穩(wěn)定性以及郵寄前后的影響。結(jié)果與普通樣品管比較,采集器保存的尿液,對(duì)HIV-1抗體檢測(cè)無(wú)明顯影響(P=0.24);在不同溫度下,采集器可穩(wěn)定保存尿液的時(shí)間:采集器尿液在-20℃和4℃保存1年,在常溫保存1個(gè)月,在37℃保存9天。均一性:批間和批內(nèi)變異系數(shù)(CV)均15%(P0.05);郵寄前后尿液HIV-1抗體無(wú)顯著變化,Kappa值為0.957。結(jié)論一次性尿液采集器可用于檢測(cè)HIV-1抗體尿液樣本的采集、貯存與運(yùn)輸。
[Abstract]:Objective to evaluate the feasibility of using disposable urine collector to detect HIV antibody. Methods the effects of ordinary sample tubes and collectors on the detection of HIV-1 antibody in urine were compared by Elisa, and the homogeneity of HIV-1 antibody in urine was further examined at different temperature and storage time. Stability and influence before and after mailing. Results compared with the normal sample tube, the urine stored by the collector had no obvious effect on the detection of HIV-1 antibody, and the time of the urine retention was stable at different temperatures: the urine was stored at -20 鈩,

本文編號(hào):1932561

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