發(fā)布時(shí)間：2018-05-24 16:42

  本文選題：干燥綜合征 + 抗SSA抗體��； 參考：《山東醫(yī)藥》2017年25期

【摘要】：目的觀察抗SSA抗體陽(yáng)性干燥綜合征(p SS)患者血清miR-34、miR-500表達(dá)變化,并探討二者在抗SSA抗體陽(yáng)性p SS診斷中的臨床價(jià)值。方法收集p SS患者75例(觀察組),非p SS患者84例(對(duì)照組),miRNA芯片分析兩組血清miRNA表達(dá)譜改變;并通過(guò)實(shí)時(shí)熒光定量RT-PCR進(jìn)行驗(yàn)證。隨后應(yīng)用統(tǒng)計(jì)學(xué)方法分析異常表達(dá)miRNA在抗SSA抗體陽(yáng)性p SS診斷中的價(jià)值。結(jié)果 miRNA芯片分析發(fā)現(xiàn)6個(gè)上調(diào)和4個(gè)下調(diào)的miRNA,其中miR-34和miR-500表達(dá)在觀察組血清中明顯升高;實(shí)時(shí)熒光定量PCR的結(jié)果證實(shí)miR-34和miR-500在觀察組明顯上調(diào),與對(duì)照組比較,P0.001。受試者工作曲線分析結(jié)果顯示,觀察組miR-34對(duì)p SS診斷的AUC為0.783(95%CI:0.710~0.844,P0.001);miR-500的AUC為0.774(95%CI:0.701~0.837,P0.001)。而miR-34聯(lián)合miR-500在p SS診斷中AUC為0.888(95%CI:0.828~0.932,P0.001);兩者聯(lián)合診斷p SS的AUC與單一檢測(cè)比較,P均0.05。結(jié)論 miR-34和miR-500在抗SSA抗體陽(yáng)性p SS患者血清中表達(dá)升高,檢測(cè)血清miR-34和miR-500有助于抗SSA抗體陽(yáng)性p SS診斷。
[Abstract]:Objective to observe the changes of serum miR-34 miR-500 expression in patients with anti SSA antibody positive Sjogren's syndrome (SSS) and to explore their clinical value in the diagnosis of anti SSA antibody positive PSS. Methods 75 patients with PSS (observation group, 84 patients without PSS) were collected. The changes of serum miRNA expression profiles in two groups were analyzed by miRNA microarray and verified by real-time fluorescence quantitative RT-PCR. Then the value of abnormal expression of miRNA in the diagnosis of anti SSA antibody positive PSS was analyzed by statistical method. Results six upregulated miRNAs and 4 down-regulated miRNAs were found by miRNA microarray analysis, in which the expression of miR-34 and miR-500 was significantly increased in the serum of the observation group, and the results of real-time fluorescence quantitative PCR confirmed that miR-34 and miR-500 were up-regulated in the observation group, compared with the control group (P 0.001). The results of the operating curve analysis showed that the AUC of miR-34 for PSS in the observation group was 0.783 / 95 and the AUC of the observation group was 0.774 / 95CI: 0.701and 0.837 / P0.001respectively. In the diagnosis of PSS, the AUC of miR-34 combined with miR-500 was 0. 888 + 95% CI: 0. 828 and 0. 932% P0.001, and the AUC of the combined diagnosis of PSS was both 0. 05% and 0. 05% compared with that of single detection (P < 0. 05). Conclusion the expression of miR-34 and miR-500 in serum of patients with anti SSA antibody positive PSS is increased. Detection of serum miR-34 and miR-500 is helpful for the diagnosis of anti SSA antibody positive PSS.
【作者單位】： 江南大學(xué)附屬醫(yī)院·無(wú)錫市第四人民醫(yī)院;無(wú)錫市人民醫(yī)院;杭州師范大學(xué)附屬醫(yī)院;
【基金】：亞太風(fēng)濕病學(xué)會(huì)聯(lián)盟基金資助項(xiàng)目(2011) 江蘇省自然科學(xué)基金青年項(xiàng)目(BK20140171)
【分類(lèi)號(hào)】：R593.2

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