發(fā)布時間：2018-05-11 00:35

  本文選題：基因多態(tài)性 + 高血脂��； 參考：《上海體育學(xué)院》2016年碩士論文

【摘要】：研究目的通過查閱文獻(xiàn),選取了7個與血脂及運動相關(guān)的SNP位點(APOA5基因rs662799、AVPV1α基因rs1042615、GHRL基因rs26802、NOS3基因rs2070744以及IL基因上的rs2228059、rs1589241和rs1800795),欲在長期進(jìn)行有氧運動的老年人群中,探討這些位點與高脂血癥的關(guān)聯(lián)性,并針對有關(guān)聯(lián)的位點,分層研究不同基因型與體力活動對血脂水平的影響作用,從而可以為高血脂患者制定個性化的運動處方提供科學(xué)的理論依據(jù)。研究方法從上海市楊浦區(qū)、虹口區(qū)、徐匯區(qū)、浦東新區(qū)四個轄區(qū)的部分社區(qū)招募有長期規(guī)律運動史的老年人。納入標(biāo)準(zhǔn):(1)年齡介于60~69歲;(2)身體健康,無嚴(yán)重心腦血管疾病且近期未接受調(diào)脂類藥物治療;(3)規(guī)律運動史:從事規(guī)律運動鍛煉3年以上,且每次鍛煉時間不低于30min,每周運動次數(shù)不少于3次。通過初篩問卷和進(jìn)一步體格檢查,最終納入符合條件的受試者共計432例,剔除部分信息缺失者9例后,實際有效受試者共計423例。其中男性182例,女性241例,最小年齡60歲,最大年齡69歲,平均年齡63.5±3.1歲。采集受試者空腹靜脈血,分別采用膽固醇氧化酶法、GPO-POD酶法及酶促顯色法測定膽固醇、甘油三酯和低高密度脂蛋白膽固醇的水平,并對受試者進(jìn)行基本的體格測試(身高、體重、BMI等)。根據(jù)2007年制定的《中國成人血脂異常防治指南》血脂異常的界定標(biāo)準(zhǔn),結(jié)合問卷和血脂測定結(jié)果,把受試者分為高血脂組和血脂正常組(對照組)。應(yīng)用基質(zhì)輔助激光解吸附電離飛行時間質(zhì)譜檢測技術(shù)(MALDI-TOF)對預(yù)先挑選的影響脂代謝的多態(tài)位點進(jìn)行基因分型。體力活動水平通過《國際體力活動量表(International Physical Activity Questionnaire,IPAQ)中文版長卷》進(jìn)行測量,并按照IPAQ制定的標(biāo)準(zhǔn),計算每周體力活動能量消耗的MET值。研究結(jié)果1.高血脂組的總膽固醇、甘油三酯、低密度脂蛋白水平均顯著高于對照組(P0.001),而高密度脂蛋白水平則顯著低于對照組(P0.001),兩組間的體重指數(shù)、血壓、血糖及各體力活動水平的人群分布均沒有統(tǒng)計學(xué)差異(P0.05)。2.除了rs1800795位點未發(fā)現(xiàn)多態(tài)性外,其他6個位點均符合Hardy-Weinberg平衡定律(P0.05),僅APOA5基因rs662799位點的基因型及等位基因頻率在高血脂組和對照組中的分布差異有統(tǒng)計學(xué)意義(χ2=11.233,P=0.004,χ2=11.698,P=0.001),其他5個多態(tài)位點在兩組間的分布情況均未表現(xiàn)出統(tǒng)計學(xué)差異(P0.05)。3.利用多元logistic回歸分析,校正了性別、年齡、BMI及體力活動水平后發(fā)現(xiàn),rs662799位點的三種基因型中,AG和GG基因型攜帶者患高血脂的風(fēng)險分別是AA基因型的1.676倍(P=0.018,95%CI:1.092-2.571)和1.812倍(P=0.002,95%CI:1.247-2.632),進(jìn)一步利用線性回歸分析rs662799與血脂水平的關(guān)聯(lián)性發(fā)現(xiàn),G等位基因的存在與TG水平呈顯著正相關(guān)(P0.001)、與HDL水平顯著負(fù)相關(guān)(P=0.001)。單因素方差分析結(jié)果顯示,AA、AG和GG基因型個體的TG水平依次升高,分別為1.30±0.56mmol/L、1.68±0.82mmol/L和1.99±0.94 mmol/L,且AG和GG基因型個體的水平均顯著高于AA基因型個體(P0.01);而AA、AG和GG基因型個體的HDL水平依次降低,分別為1.43±0.37 mmol/L、1.36±0.31 mmol/L和1.28±0.28 mmol/L,且GG基因型個體的水平顯著高于AA基因型個體(P0.05)。4.利用四分位法將體力活動水平進(jìn)行分級后發(fā)現(xiàn),受rs662799基因多態(tài)性的影響,體力活動與TG水平的關(guān)系不同:AA基因型攜帶人群,在不同體力活動水平下,TG水平均未有統(tǒng)計學(xué)差異(P0.05),而AG+GG基因型攜帶人群在不同體力活動水平下,TG水平具有顯著性差異(P0.05),當(dāng)體力活動水平高于8134.5 MET-min/w時,TG出現(xiàn)了最低水平(1.45±0.74)mmol/L,較體力活動水平低于5460 MET-min/w時顯著降低(P0.05)。研究結(jié)論1.APOA5基因rs662799單核苷酸多態(tài)性與長期有氧鍛煉老年人的高血脂存在密切關(guān)聯(lián),G等位基因可能是高血脂發(fā)生的易感基因。2.體力活動與血脂水平的關(guān)系可能受到rs662799基因多態(tài)性的影響,AA基因型攜帶人群的TG水平受體力活動的影響不大,而AG和GG基因型人群可能在較高水平(PA8134.5 MET-min/W)的體力活動下,可以有效改善TG水平。
[Abstract]:The purpose of this study was to select 7 SNP loci related to blood lipid and exercise (APOA5 gene rs662799, AVPV1 a gene rs1042615, GHRL gene rs26802, NOS3 gene rs2070744 and IL gene rs2228059, rs1589241, and IL). The effects of different genotypes and physical activity on the level of blood lipids were studied in stratification, which could provide scientific theoretical basis for the formulation of personalized exercise prescriptions for hyperlipidemia patients. The research methods were recruited from four communities in Yangpu District, Hongkou District, Xuhui District and Pudong New Area, Shanghai. The elderly with a history of regular exercise history included standard: (1) age between 60~69 years; (2) healthy, no serious cardio cerebral vascular disease and no lipo therapy in the near future; (3) regular exercise history: regular exercise for more than 3 years, and each exercise is not less than 30min, no less than 3 times a week. Through the initial screening questionnaire And further physical examination, a total of 432 eligible subjects were included. After 9 cases of missing information, the total number of effective subjects was 423 cases, including 182 males and 241 females, the minimum age of 60 years, the maximum age of 69, and the average age of 63.5 + 3.1 years. The levels of cholesterol, triglycerides, and low density lipoprotein cholesterol were measured by GPO-POD enzyme method and enzymatic colorimetric method, and the subjects were tested with basic physical tests (height, weight, BMI, etc.). According to the guidelines for the prevention and control of dyslipidemia in Chinese adults and the criteria for the determination of blood lipids in 2007, the results of the questionnaire and blood lipid determination were combined. The subjects were divided into hyperlipidemia group and normal blood lipid group (control group). Using matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF), the pre selected polymorphic loci affecting lipid metabolism were genotyping. The level of physical activity was measured by the international physical activity scale (International Physical Activity Questionnaire, IP). AQ) the Chinese version of long scroll > measured and calculated the MET value of energy consumption of physical activity per week according to the standard set by IPAQ. Results the total cholesterol, triglyceride, low density lipoprotein level of the 1. hyperlipidemia group were significantly higher than that of the control group (P0.001), while the high density lipoprotein level was significantly lower than that of the control group (P0.001), and the weight of the two groups between the high blood fat groups was significantly lower than that of the control group (P0.001). The distribution of index, blood pressure, blood glucose and the level of physical activity were not statistically different (P0.05).2., except for the polymorphism of rs1800795 loci, the other 6 loci were consistent with the Hardy-Weinberg equilibrium law (P0.05), and the distribution of genotype and allele frequency in the rs662799 locus of APOA5 gene in the hyperlipidemia group and the control group was poor. The difference was statistically significant (x 2=11.233, P=0.004, X 2=11.698, P=0.001), and the distribution of the other 5 polymorphic loci in the two groups was not statistically significant (P0.05).3. using multiple logistic regression analysis to correct the sex, age, BMI and physical activity levels, and the three genotypes of the rs662799 loci, AG and GG, were carried. The risk of hyperlipidemia was 1.676 times (P=0.018,95%CI:1.092-2.571) and 1.812 times (P=0.002,95%CI:1.247-2.632) of the AA genotype, respectively. Further using linear regression analysis the correlation between rs662799 and blood lipid levels, the existence of G allele was significantly positively correlated with the level of TG (P0.001), and was negatively correlated with the level of HDL (P=0.001). The results of the prime variance analysis showed that the levels of TG in AA, AG and GG genotype individuals were increased in turn, 1.30 + 0.56mmol/L, 1.68 + 0.82mmol/L and 1.99 + 0.94 mmol/L respectively, and the levels of AG and GG genotype individuals were significantly higher than those of AA genotype individuals (P0.01), while AA, 1.43 + 0.37, 1.36, 1.36, respectively, 1.36, 1.36, respectively. 1.36 0.31 mmol/L and 1.28 + 0.28 mmol/L, and the level of GG genotype individuals was significantly higher than that of AA genotype individuals (P0.05).4. using four division method to classify the level of physical activity. The relationship between physical activity and TG level was different from that of rs662799 gene polymorphism: AA based type carrying population, TG water at different physical activity levels. There was no statistical difference (P0.05) on average (P0.05), while AG+GG genotype had significant difference (P0.05) at different levels of physical activity (P0.05). When the level of physical activity was higher than 8134.5 MET-min/w, TG had the lowest level (1.45 + 0.74) mmol/L, which was significantly lower than that of physical activity below 5460 MET-min/w (P0.05). Conclusions 1. The single nucleotide polymorphism of the APOA5 gene rs662799 is closely related to the high blood lipid in the elderly with long-term aerobic exercise. The G allele may be a susceptible gene of hyperlipidemia, the relationship between the physical activity of.2. and the blood lipid level may be affected by the polymorphism of the rs662799 gene. The TG level of the AA genotype group is not affected by physical activity. Large and AG and GG genotypes may be effective at improving TG levels at higher levels (PA8134.5 MET-min/W).

【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R589.2
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本文編號：1871648