發(fā)布時(shí)間：2018-05-13 17:48

  本文選題：產(chǎn)糖脂表面活性劑菌 + 篩選　； 參考：《微生物學(xué)通報(bào)》2017年11期

【摘要】：【目的】從油頁(yè)巖環(huán)境中篩選可降解油頁(yè)巖干酪根的產(chǎn)生物表面活性劑菌株�！痉椒ā繌膿犴樣晚�(yè)巖礦廢水樣品中用血平板法初篩,排油圈法、乳化法和表面張力法復(fù)篩,獲得產(chǎn)生物表面活性劑菌株。對(duì)目標(biāo)菌株進(jìn)行生理生化鑒定、16S r RNA基因序列和系統(tǒng)發(fā)育分析,用薄層色譜鑒定其發(fā)酵液表面活性成分,優(yōu)化產(chǎn)表面活性劑的培養(yǎng)條件,初步考察其對(duì)油頁(yè)巖干酪根的降解能力。【結(jié)果】篩選到一株產(chǎn)糖脂表面活性劑菌株B-1,初步鑒定為Pseudomonas sp.,該菌株有良好的排油和乳化能力以及較低的表面張力,可利用烷烴、不飽和脂肪酸和糖類作為碳源。在30-34°C范圍內(nèi)添加0.3%Na Cl的葡萄糖培養(yǎng)基(p H 7.0)中該菌生長(zhǎng)旺盛,發(fā)酵液表面張力最低為27 m N/m。菌株B-1在添加一定量葡萄糖的無(wú)機(jī)鹽培養(yǎng)基中作用30 d后對(duì)干酪根的降解率為2.85%,高于不添加葡萄糖無(wú)機(jī)鹽培養(yǎng)基對(duì)照組的降解率(1.04%)�！窘Y(jié)論】菌株B-1是一株性能良好的產(chǎn)糖脂表面活性劑細(xì)菌,有降解干酪根的潛力。
[Abstract]:[objective] to screen biodegradable kerogen surfactant strains from oil shale environment. [methods] Blood plate method was used to screen the samples from Fushun oil shale waste water, oil discharge circle method, emulsification method and surface tension method were used to screen again. The strain producing surfactant was obtained. The 16s r RNA gene sequence and phylogenetic analysis of the target strain were carried out. The surface active components of the fermentation broth were identified by TLC, and the culture conditions of surfactant production were optimized. [results] A saccharide-producing surfactant strain B-1 was selected and identified as Pseudomonas sp., which has good oil removal and emulsifying ability and low surface tension, so it can utilize alkanes. Unsaturated fatty acids and sugars are used as carbon sources. In the glucose medium supplemented with 0.3%Na Cl in the range of 30-34 擄C (pH 7.0), the growth of the strain was vigorous, and the surface tension of the fermentation broth was the lowest 27 m N / m. The degradation rate of kerogen of strain B-1 in inorganic salt medium supplemented with a certain amount of glucose for 30 days was 2.85, which was higher than that of control group without glucose. [conclusion] strain B-1 is a good strain. Good glycolipid surfactant bacteria, It has the potential to degrade kerogen.
【作者單位】： 東北大學(xué)生命科學(xué)與健康學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.51204055) 國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(No.160096)~~
【分類號(hào)】：Q939.97;TE662
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本文編號(hào)：1884183