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大鼠腦外傷后BDNF的表達(dá)及其死后穩(wěn)定性的研究

發(fā)布時(shí)間:2018-07-04 21:13

  本文選題:法醫(yī)病理學(xué) + 免疫組織化學(xué); 參考:《重慶醫(yī)科大學(xué)學(xué)報(bào)》2009年11期


【摘要】:目的:探討腦外傷后腦源性神經(jīng)營(yíng)養(yǎng)因子(Brain derived neurotrophical factor,BDNF)表達(dá)的變化和損傷時(shí)間的關(guān)系及其死后穩(wěn)定性。方法:將健康SD大鼠105只,隨機(jī)分成4組:正常組、假手術(shù)組、腦外傷組、死后穩(wěn)定性組。采用重物砸傷腦組織建立腦外傷模型(創(chuàng)傷組、穩(wěn)定性組),運(yùn)用免疫組化和圖像分析技術(shù),檢測(cè)各組BDNF的表達(dá)變化規(guī)律。結(jié)果:與正常組、假手術(shù)組比較,損傷后0.5 h,BDNF的免疫反應(yīng)陽(yáng)性細(xì)胞在損傷區(qū)周?chē)龆?損傷后6 h BDNF陽(yáng)性細(xì)胞數(shù)達(dá)高峰,以后逐漸下降,24 h后降至接近正常;死后穩(wěn)定性組各時(shí)間點(diǎn)BDNF表達(dá)無(wú)顯著性差異(P0.05)。結(jié)論:腦外傷后BDNF表達(dá)與損傷時(shí)間具有相關(guān)性;在-20℃條件下,死后穩(wěn)定性好。
[Abstract]:Aim: to investigate the relationship between the expression of brain-derived neurotrophic factor (BDNF) and the time of injury and postmortem stability after traumatic brain injury. Methods: 105 healthy SD rats were randomly divided into 4 groups: normal group, sham operation group, brain injury group and postmortem stability group. The brain injury model (trauma group, stability group) was established by heavy object injury. The expression of BDNF in each group was detected by immunohistochemistry and image analysis. Results: compared with normal group and sham operation group, the number of BDNF immunoreactive cells increased around the injured area at 0.5 h after injury, reached the peak at 6 h after injury, then decreased to almost normal at 24 h after injury. There was no significant difference in BDNF expression at all time points in postmortem stability group (P0.05). Conclusion: the expression of BDNF is correlated with the time of injury, and the postmortem stability is good at-20 鈩,

本文編號(hào):2097445

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