發(fā)布時間：2018-05-25 00:10

  本文選題：乙型肝炎病毒 + 基因型�。� 參考：《現(xiàn)代預防醫(yī)學》2017年07期

【摘要】：目的明確石家莊地區(qū)乙肝基因型/亞型分布特征,探索其與乙肝感染后疾病進展的相關(guān)性。方法采用型特異性引物多重聚合酶鏈反應(PCR)方法對270例乙肝表面抗原(HBsAg)陽性慢性乙肝、肝硬化和原發(fā)性肝癌患者血清標本進行HBV基因型/亞型檢測,應用SPSS16.0進行統(tǒng)計描述和檢驗。結(jié)果基因型檢測成功樣本共233例,其中,C型占81.11%(189/233),B型占18.03%(42/233),D型占0.86%(2/233),基因亞型檢測結(jié)果為:C2亞型占75.10%(175/233),B2亞型占16.74%(39/233),C1亞型占6.01%(14/233),B1亞型占1.29%(3/233);原發(fā)性肝癌、慢性乙肝患者中C基因型比例顯著高于肝硬化(P=0.013,P=0.026);C基因型HBV感染者相對B型具有更高的HBe Ag陽性率和HBV DNA滴度(P=0.012,P=0.002);相對B型,C型HBV患者更易進展為肝硬化失代償期(P=0.006),且具有顯著更高的患癌風險(P=0.029)。結(jié)論石家莊地區(qū)流行HBV以C型和B型為主,其中C2、B2為主要亞型,HBV C型感染者更容易進展為失代償肝硬化,發(fā)生肝癌的風險更高,但是需要更大樣本量研究進行驗證。
[Abstract]:Objective to determine the distribution of hepatitis B genotype / subtype in Shijiazhuang area and to explore its correlation with the progression of hepatitis B infection. Methods HBV genotypes / subtypes were detected by type specific primer polymerase chain reaction (PCR) in 270 serum samples from patients with chronic hepatitis B, liver cirrhosis and primary liver cancer. Use SPSS16.0 to describe and test statistics. Results there were 233 successful genotypic samples, of which 81.11c / 81.113B / 18.03 / 42 / 233C / D / 0.86 / 233C, and 75.101055 / 233B2 / 75.101055 / 233B2 / 16.7474 / 393233C _ 1 / 6.01p / 233mb _ 1 / 1.29 / 233C ~ (3 / 23); Primary liver cancer, respectively, = 1.29% / 2333 / 23 ~ (3 / 23); Primary liver cancer,% C _ 2 = 175.75% / 233B ~ (-1) = 16.74 / 39 / 23 ~ 3 / C ~ 1 = 6.01% / 23 / 23 ~ (-1). The ratio of genotype C to genotype C was significantly higher in patients with chronic hepatitis B than that in patients with liver cirrhosis. The positive rate of HBe Ag and the titer of HBV DNA in patients with HBV were higher than those in patients with type B HBV, and the patients with type B HBV were more likely to progress to the stage of decompensation of liver cirrhosis (P0.006). And the risk of cancer was significantly higher than that of P0. 029. Conclusion the prevalence of HBV in Shijiazhuang is mainly of type C and B, in which type C infection of C2B 2 is more likely to develop into decompensated cirrhosis, and the risk of liver cancer is higher, but larger sample size is needed to verify it.
【作者單位】： 白求恩醫(yī)務士官學校預防醫(yī)學教研室;白求恩國際和平醫(yī)院檢驗科;
【基金】：河北省自然科學基金(H2015509001)
【分類號】：R512.62

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